Technology

Mastitis Pathogen Identification Techniques/TLRI

This technology refers to the design of primers based on the DNA sequences of 15 common bacterial species found in raw milk, such as Staphylococcus aureus, Mycoplasma bovis, and Enterococcus spp., as published on the NCBI website. Real-time quantitative PCR (qPCR) technology is then used for detection.The qPCR, which has predefined cycle threshold (Ct) values and internal controls allowing differentiation between truly positive and false negative results, has proved useful to identify mastitis pathogens. It is based on identification of bacterial genome sequences, and can detect growth inhibited and dead bacteria within 3 to 4 hours.
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