Enzyme-Linked Immunosorbent Assay (ELISA) Diagnostic Reagent for the Non-Structural Protein Antibody Detection to Foot-and-Mouth Disease Virus/VRI
|
Sandwich enzyme-linked immunosorbent assay (ELISA) based assays can be used to detect antibodies to non-structural proteins of FMD virus O serotype. We have used the prokaryotic expression system for producing non-structural proteins and set up a sandwich ELISA to detect antibodies to the non-structural proteins. After testing 770 sera samples, our results have revealed that the specificity and sensitivity of our ELISA could be up to 100 ％ and 98.4 ％, respectively. Moreover, by comparing to the tested result, we have found out that the diagnostic sensitivity of our ELISA is higher than that of Escherichia coli commercial ELISA kit (35.5％). Moreover, it can also detect antibodies to non-structural proteins of serotypes A, C, Asia 1, SAT 1, SAT 2, and SAT 3 in bovine sera. Furthermore, the absence of cross-reactions generated by different antibody titers against swine vesicular disease virus also highlighted the assay’s specificity. |
|
|
|
|